- Solvent selection is a key step in the method development process for DESI. Always consider the analyte chemistry when choosing a solvent composition. Follow electrospray ionization principles.
- Spray stability is a must in order to achieve reproducible measurements using DESI. Ensure that the solvent flow rate is stable, the DESI emitter is free of debris and cracks, the solvent delivery line is not pinched restricting flow and there are no air bubbles in the line or syringe.
- The spot size increases and decreases based on the solvent flow rate and the nebulization gas pressure. Be aware that as the spot size decreases, a corresponding decrease in sensitivity occurs.
- DESI spray voltages above 4kV are not recommended. Discharging will occur that is deleterious to the analyte signal. The working range is 2-3.5kV.
- Continuous velocity scanning is recommended for most applications. In the DESI Motion Control software, use Start Point CV.
Monday, November 5, 2012
5 tips for success in DESI imaging
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