New Publication: Localization and Quantification of Drugs in Animal Tissues Using Desorption Electrospray Ionization Mass Spectrometry Imaging

DESI imaging using an Orbitrap mass analyzer in full scan mode was performed on 6 µm coronal brain sections from rats that were administered 2.5 mg/kg Clozapine. Clozapine was localized and quantified in individual brain sections 45 minute post-dose. External calibration curves were prepared by micropipetting standards with internal standard (IS) on top of the tissues and average response factors were calculated for the scans which both Clozapine and IS were detected. All response factors were normalized to area units. Quantifications from DESI imaging revealed 0.2 ng -1.2 ng Clozapine in individual brain sections which results were further confirmed with extraction and LC/MS/MS Analysis.

http://pubs.acs.org/doi/abs/10.1021/ac3011654